Masirinji Anoraswa - Appendix 2

Majekiseni Anoraswa - Appendikisi II

1. Bacterial endotoxin bvunzo:

1.1 Kugadzirira bvunzo:

Midziyo inoshandiswa mukuedzwa inoda kurapwa.Nzira yakajairika ndeyekuomesa kubika pa180 ℃ kwemaawa maviri.Kusvibiswa kweMicrobial kuchadzivirirwa panguva yekuedzwa kushanda.

Mvura yebhakitiriya endotoxin bvunzo inoreva mvura yakasvava yejekiseni isingaburitse condensation reaction neLAL reagent ine sensitivity ye 0.03EU/ml kana kukwira pasi pe37 ℃± 1 ℃ kwemaawa makumi maviri nemana.

1.2 Nzira yekuedza:

Tora 8 maampoules epakutanga e0.1 ml/chidimbu che lysate lysate, 2 ayo anowedzerwa ne 0.1 ml yemhinduro yebvunzo sechubhu yekuyedza, uye maviri ayo anowedzerwa ne 0.1 ml ye2.0 yakagadzirwa nechiyero chekushanda chebhakitiriya endotoxin nemvura. yebhakitiriya endotoxin bvunzo λ Iyo yekumisikidza endotoxin mhinduro inoshandiswa seyakanaka yekudzora chubhu.Wedzera 0.1ml yebhakitiriya endotoxin yekuyedza mvura kune maviri machubhu seyakaipa yekudzora chubhu.Wedzera 0.1ml yechinyorwa chebvunzo chakanakira kudzora mhinduro kune maviri machubhu λ Concentration ye endotoxin mhinduro] seyakanaka yekudzora chubhu yechinyorwa chebvunzo.Zvinyoro sanganisa mhinduro muchubhu yekuyedza, vhara muromo, woiisa mubhokisi remvura yekugezera 37 ℃± 1 ℃, uye zvinyoro nyoro ibudise mushure me60 ± 2min yekuchengetedza kupisa.Dzivisa mhedzisiro yenhema inokonzerwa nekudengenyeka panguva yekuchengetedza kupisa uye kutora chubhu.

Mutongo wemhedzisiro:

Zvinyoro tora chubhu yekuedza uye zvishoma nezvishoma uitenderedze pasi kune 1800. Kana iyo gel muchubhu isina kuremara uye isingatsvedze kubva pamadziro echubhu, imhedzisiro yakanaka uye yakanyorwa se (+);Kana iyo gel isingagoni kuchengetwa yakasimba uye inotsvedza kubva pamadziro echubhu, mhedzisiro yakaipa inonyorwa se (-).