Amasirinji Alahlwayo - Isithasiselo II
1. Ukuhlolwa kwe-bacterial endotoxin:
1.1 Ukulungiselela ukuhlolwa:
Izitsha ezisetshenziswe ekuhlolweni zidinga ukwelashwa.Indlela evamile ukumisa ukubhaka ku-180 ℃ amahora angu-2.Ukungcoliswa kwamagciwane kuzovinjelwa ngesikhathi sokuhlolwa.
Amanzi okuhlolwa kwe-endotoxin yebhaktheriya abhekisela emanzini abulawe inzalo omjovo angakhiqizi ukujiya kokujiya nge-LAL ezwelayo okungu-0.03EU/ml noma ngaphezulu ngaphansi kuka-37 ℃± 1 ℃ amahora angu-24.
1.2 Indlela yokuhlola:
Thatha ama-ampoule angu-8 okuqala ka-0.1 ml/ucezu lwe-lysate lysate, ama-2 awo engezwa no-0.1 ml wesisombululo sokuhlola njengeshubhu lokuhlola, futhi ama-2 awo engezwa ngo-0.1 ml ka-2.0 enziwe ngezinga lokusebenza le-endotoxin yebhaktheriya ngamanzi. ukuhlolwa kwe-endotoxin yebhaktheriya λ Ukugxila kwesisombululo se-endotoxin kusetshenziswa njengeshubhu lokulawula elihle.Engeza u-0.1ml wamanzi okuhlola i-endotoxin yebhaktheriya kumashubhu ama-2 njengeshubhu lokulawula elibi.Engeza u-0.1ml we-athikili yokuhlola isixazululo sokulawula esihle kumashubhu angu-2 λ Ukugxiliswa kwesisombululo se-endotoxin] njengeshubhu lokulawula elivumayo le-athikili yokuhlola.Xuba ngobumnene isixazululo eshubhuni yokuhlola, vala umlomo wombhobho, uwubeke ubheke phezulu ebhokisini lokugezela lamanzi elingu-37 ℃± 1 ℃, bese ulikhipha ngobumnene ngemva kwama-60 ± 2min wokulondoloza ukushisa.Gwema imiphumela emihle engamanga ebangelwa ukudlidliza ngesikhathi sokulondoloza ukushisa nokuthatha amashubhu.
Ukwahlulela komphumela:
Khipha ngobumnene ishubhu lokuhlola bese ulibhekisa phansi kancane kancane ku-1800. Uma ijeli eshubhu ingakhubazekile futhi ingasheleli odongeni lweshubhu, kuwumphumela omuhle futhi urekhodwe njengo (+);Uma ijeli ingakwazi ukugcinwa iphelele futhi ishelela odongeni lweshubhu, umphumela ongemuhle ubhalwa ngokuthi (-).
(1) Amashubhu okuhlola amabili (-), okufanele abhekwe njengahlangabezana nezimfuneko;Uma kokubili kungu-(+), kuzothathwa njengokungafaneleki.
(2) Uma ishubhu elilodwa lokuhlola lithi (+) elinye lithi (-), thatha amanye amashubhu okuhlola amane ukuze uwahlole kabusha ngokwendlela engenhla, futhi elinye kwamane lithi (+), libhekwa njengelingafanelekile.
(3) Uma ishubhu lokulawula eliphozithivu lithi (-) noma isampula yokuhlola ithi (-) noma ishubhu lokulawula elinegethivu lithi (+), ukuhlola akuvumelekile.
Amasirinji Alahlwayo - Isithasiselo III
1. Uhlelo lwesampula olulodwa lwamukelwa ukuze luhlolwe ngezikhathi ezithile.Ukuhlukaniswa kwemikhiqizo engahambisani, iqembu lokuhlola, izinto zokuhlola, izinga lokubandlulula, i-RQL (izinga lekhwalithi elingavumelani) nohlelo lwamasampula kucaciswe kuthebula elilandelayo.
Qaphela: Okuqukethwe kwe-cadmium ku-5.14.1 kwezinga kuphathiswe ukuthi kuhlolwe.
2. Uhlelo lwesampula lwesikhathi esisodwa lwamukelwa ukuze luhlolwe iqoqo.Ukuqina kuqala ohlelweni olujwayelekile lokuthatha amasampula.Ukuhlukaniswa, izinto zokuhlola, izinga lokuhlola (IL) kanye neleveli yekhwalithi efanelekayo (AQL) yemikhiqizo engavumelani kucaciswe kuthebula elilandelayo.
Isikhathi sokuthumela: Oct-06-2022
