Syringes enwere ike iwepu - Ihe mgbakwunye 2

Syringes enwere ike iwepụ - Ihe Odide II

1. Nnwale endotoxin nke nje bacteria:

1.1 Nkwadebe ule:

Ọ dị mkpa ka a gwọọ arịa ndị a na-eji n'ule.Usoro a na-emekarị bụ ịkpọ nkụ na 180 ℃ maka awa 2.A ga-egbochi mmetọ microbial n'oge ọrụ nnwale.

Mmiri maka ule endotoxin nke nje bacteria na-ezo aka na mmiri igba ogwu maka injection nke na-adịghị emepụta mmeghachi omume condensation na LAL reagent nwere mmetụta nke 0.03EU/ml ma ọ bụ karịa n'okpuru 37 ℃± 1 ℃ maka awa 24.

1.2 Usoro nnwale:

Were 8 ampoules mbụ nke 0.1 ml / mpempe lysate lysate, 2 n'ime ha na-agbakwunye ya na 0.1 ml nke ngwọta ule dị ka tube ule, na 2 n'ime ha na-agbakwunye 0.1 ml nke 2.0 nke ụkpụrụ ọrụ nke endotoxin nje na mmiri. maka ule endotoxin nje bacteria λ A na-eji ntinye nke ngwọta endotoxin mee ihe dị ka tube njikwa dị mma.Tinye 0.1ml nke mmiri ule endotoxin nje na tubes 2 dị ka tube njikwa na-adịghị mma.Tinye 0.1ml nke ule nyocha ihe ngwọta nchịkwa dị mma na tubes 2 λ Ntụkwasị obi nke ngwọta endotoxin] dị ka tube njikwa ziri ezi nke isiokwu ule.Jiri nwayọọ gwakọta ihe ngwọta na tube ule, mechie nozzle, kwụ ọtọ tinye ya n'ime igbe ịsa mmiri 37 ℃ 1 ℃, ma jiri nwayọọ wepụ ya mgbe 60 ± 2min nke nchekwa okpomọkụ gasịrị.Zere nsonaazụ dị mma nke ụgha kpatara site na ịma jijiji n'oge nchekwa okpomọkụ yana tube.

Mkpebi nsonaazụ:

Jiri nwayọọ wepụ tube ule ahụ ma jiri nwayọọ nwayọọ gbanwee ya maka 1800. Ọ bụrụ na gel na tube adịghị edozi ma ghara ịdaba na mgbidi tube, ọ bụ nsonaazụ dị mma ma dekọọ dị ka (+);Ọ bụrụ na gel enweghị ike idobe ya ma na-apụ na mgbidi tube, a na-edekọ ihe ọjọọ dị ka (-).