Imiti ikoreshwa - Umugereka wa 2

Imiti ikoreshwa - Umugereka wa II

1. Ikizamini cya bagiteri endotoxine:

1.1 Gutegura ikizamini:

Imiyoboro ikoreshwa mu kizamini igomba kuvurwa.Uburyo busanzwe nukumisha guteka kuri 180 ℃ kumasaha 2.Kwanduza mikorobe bigomba gukumirwa mugihe cyo gukora ibizamini.

Amazi yo kwisuzumisha ya bagiteri ya endotoxine yerekeza kumazi ya sterisile yo gutera inshinge idatanga reaction ya LAL reagent hamwe na sensibilité ya 0.03EU / ml cyangwa irenga munsi ya 37 ℃ ± 1 ℃ mumasaha 24.

1.2 Uburyo bw'ikizamini:

Fata ampules 8 yumwimerere ya 0.1 ml / igice cya lysate lysate, 2 muri yo ikongerwamo 0.1 ml yumuti wikizamini nkumuyoboro wikizamini, naho 2 muri yo ikongerwamo 0.1 ml ya 2.0 ikozwe murwego rwakazi rwa bagiteri endotoxine n'amazi. kwipimisha rya bacteri endotoxin λ Ubwinshi bwumuti wa endotoxine ukoreshwa nkumuyoboro mwiza wo kugenzura.Ongeramo 0.1ml ya bagiteri endotoxine yipimisha amazi mumiyoboro 2 nkumuyoboro utari mwiza.Ongeramo 0.1ml yikizamini cyigisubizo cyiza cyo kugenzura ibisubizo 2 λ Kwibanda kumuti wa endotoxine] nkumuyoboro mwiza wo kugenzura ingingo yikizamini.Kuvanga witonze igisubizo mumiyoboro yikizamini, funga nozzle, uhagarike ubishyire mumasanduku ya 37 ℃ ± 1 ℃, hanyuma ubisohokane witonze nyuma ya 60 ± 2min yo kubika ubushyuhe.Irinde ibisubizo byiza biterwa no kunyeganyega mugihe cyo kubika ubushyuhe no gufata imiyoboro.

Urubanza:

Kuramo witonze witonze hanyuma ugerageze buhoro buhoro hejuru ya 1800. Niba gele iri muri tube idahindutse kandi ntunyerera kurukuta rwigituba, nigisubizo cyiza kandi cyanditswe nka (+);Niba gel idashobora kubikwa neza kandi iranyerera kuva kurukuta, ibisubizo bibi byanditswe nka (-).