Masyringe Otayidwa - Zowonjezera 2

Masyringe otayika - Zowonjezera II

1. Kuyesa kwa bakiteriya endotoxin:

1.1 Kukonzekera mayeso:

Zotengera zomwe zimagwiritsidwa ntchito poyesa ziyenera kuthandizidwa.Njira yodziwika ndikuwumitsa kuphika pa 180 ℃ kwa maola awiri.Kuipitsidwa kwa tizilombo toyambitsa matenda kudzapewedwa panthawi yoyeserera.

Madzi oyezetsa bakiteriya endotoxin amatanthauza madzi osabala a jakisoni omwe sapanga condensation reaction ndi LAL reagent yokhala ndi chidwi cha 0.03EU/ml kapena kupitilira apo pansi pa 37 ℃± 1 ℃ kwa maola 24.

1.2 Njira yoyesera:

Tengani ma ampoules 8 oyambilira a 0.1 ml/chidutswa cha lysate lysate, 2 mwa iwo amawonjezeredwa ndi 0,1 ml ya yankho loyesa ngati chubu choyesera, ndipo 2 mwa iwo amawonjezeredwa ndi 0,1 ml ya 2.0 yopangidwa ndi mulingo wogwira ntchito wa bakiteriya endotoxin ndi madzi. pakuyesa kwa bakiteriya endotoxin λ Kuphatikizika kwa yankho la endotoxin kumagwiritsidwa ntchito ngati chubu chowongolera chabwino.Onjezani 0.1ml yamadzi oyesa a bakiteriya endotoxin ku machubu awiri ngati chubu chowongolera.Onjezani 0.1ml ya nkhani yoyeserera yowongolera njira yabwino pamachubu 2 λ Kukhazikika kwa endotoxin solution] ngati chubu chowongolera cha nkhani yoyeserera.Sakanizani yankho mu chubu choyesera, tsekani mphuno, ikani m'bokosi losambira lamadzi la 37 ℃ ± 1 ℃, ndipo mofatsa mutulutse pambuyo pa 60 ± 2min yosungira kutentha.Pewani zotsatira zabodza zomwe zimadza chifukwa cha kugwedezeka panthawi yoteteza kutentha komanso kutenga machubu.

Zotsatira zake:

Chotsani chubu choyesera pang'onopang'ono ndikuchitembenuzira pansi kwa 1800. Ngati gel osakaniza mu chubu sakupunduka ndipo sakuchoka pakhoma la chubu, ndi zotsatira zabwino ndipo zimalembedwa ngati (+);Ngati gel osakaniza sangasungidwe bwino ndikutsika pakhoma la chubu, zotsatira zoyipa zimalembedwa ngati (-).