Iisirinji ezilahlwayo - IsiHlomelo II
1. Uvavanyo lwe-endotoxin yebhaktiriya:
1.1 Amalungiselelo ovavanyo:
Iinqanawa ezisetyenziswe kuvavanyo kufuneka ziphathwe.Indlela eqhelekileyo kukuyomisa ukubhaka kwi-180 ℃ kwiiyure ezi-2.Ungcoliseko lweMicrobial luya kuthintelwa ngexesha lomsebenzi wovavanyo.
Amanzi ovavanyo lwe-endotoxin yebhaktiriya abhekiselele kumanzi ahlambulukileyo enaliti angavelisi ukusabela kwe-condensation kunye ne-LAL reagent enovakalelo lwe-0.03EU/ml okanye ngaphezulu ngaphantsi kwe-37 ℃± 1 ℃ iiyure ezingama-24.
1.2 Indlela yovavanyo:
Thatha i-ampoules eyi-8 yokuqala ye-0.1 ml / iqhekeza le-lysate lysate, i-2 yayo yongezwa kunye ne-0.1 ml yesisombululo sovavanyo njengetyhubhu yokuvavanya, kwaye i-2 yayo yongezwa nge-0.1 ml ye-2.0 eyenziwe ngumgangatho wokusebenza we-endotoxin yebhakteria ngamanzi. kuvavanyo lwe-endotoxin yebhaktiriya λ Uxinaniso lwesisombululo se-endotoxin lusetyenziswa njengetyhubhu yokulawula elungileyo.Yongeza i-0.1ml ye-bacterial endotoxin yovavanyo lwamanzi kwiityhubhu ezi-2 njengetyhubhu yokulawula engalunganga.Yongeza i-0.1ml yenqaku lovavanyo isisombululo esilungileyo solawulo kwiityhubhu ezi-2 λ Ukugxilwa kwesisombululo se-endotoxin] njengetyhubhu yokulawula echanekileyo yenqaku lovavanyo.Xuba ngobunono isisombululo kwityhubhu yovavanyo, vala umlomo, uwubeke ngokuthe nkqo kwibhokisi yokuhlambela amanzi angama-37 ℃± 1 ℃, kwaye uyikhuphe ngobunono emva kwe-60 ± 2min yogcino lobushushu.Gwema iziphumo ezilungileyo zobuxoki ezibangelwa kukungcangcazela ngexesha lokugcinwa kobushushu kunye nokuthatha ityhubhu.
Isigwebo sesiphumo:
Thatha ngokucokisekileyo ityhubhu yokuvavanya kwaye uyijike ngokucothayo phantsi kwe-1800. Ukuba ijeli kwityhubhu ayikhubeki kwaye ayityibiliki eludongeni lombhobho, sisiphumo esihle kwaye irekhodwe njenge (+);Ukuba i-gel ayikwazi ukugcinwa ngokufanelekileyo kwaye ikhutshwe eludongeni lombhobho, umphumo ongalunganga urekhodwa njenge (-).
(1) Iityhubhu ezimbini zovavanyo zezi (-), ekufuneka zithathwe njengezihlangabezana neemfuno;Ukuba zombini ngu-(+), iya kuthathwa njengengafanelekanga.
(2) Ukuba ityhubhu yovavanyo enye ithi (+) kwaye enye ngu-(-), thatha eminye imibhobho yovavanyo emine ukuze uphinde uyivavanye ngokwendlela engasentla, kwaye enye yetyhubhu emine ngu-(+), ithathwa njengengafanelekanga.
(3) Ukuba ityhubhu yolawulo elungileyo ngu (-) okanye isampuli yovavanyo (-) okanye ityhubhu yokulawula engalunganga ngu (+), uvavanyo alusebenzi.
Iisirinji ezilahlwayo - iSihlomelo III
1. Isicwangciso sesampulu sexesha elinye samkelwa ukuze sihlolwe ngamaxesha athile.Ukuhlelwa kweemveliso ezingahambelaniyo, iqela lovavanyo, izinto zokuhlola, inqanaba localucalulo, i-RQL (inqanaba lomgangatho elingangqinelaniyo) kunye nesicwangciso sesampulu zichazwe kule theyibhile ilandelayo.
Qaphela: Umxholo we-cadmium ku-5.14.1 womgangatho uphathiswe ukuba uhlolwe.
2. Isicwangciso sesampulu sexesha elinye samkelwa ukuze kuhlolwe ibhetshi.Ubungqongqo buqala kwisicwangciso sovavanyo lwesampulu eqhelekileyo.Ukuhlelwa, izinto zokuhlola, inqanaba lokuhlola (IL) kunye nenqanaba lomgangatho ofanelekileyo (AQL) weemveliso ezingahambelaniyo zichazwe kule theyibhile ilandelayo.
Ixesha lokuposa: Oct-06-2022
